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    Generic name: 千赢国际真人


    20 samples per kit

    [Test principle]

    PSA-FITC staining was used for the shape of sperm acrosin to evaluate acrosin function. The kit can be used for the evaluation of male fertility.

    [Units of Kit]

    1.       Solution A           PSA-FITC staining solution;

    2.       Solution B           Nacl;

    3.       Solution C           BWW;

    4.       Solution D           mounting agent;

    5.       Slide                20 pieces


    Stored at -20℃ for 10 months.


    Fluorescence microscope


    Fresh sperm

    [Test method]

    (1)     One drop of fresh sperm is added into conoid test tube, then added 5 ml of physiological saline, 1000rpm×10min;

    (2)     Discard supernatant, 0.2-0.4ml, and added 1.2ml BWW culture solution, centrifuge tube beveled 45 at 37 ℃ for 1 hour;

    (3)     Erect centrifuge tube, imbibed 1 ml of upper straum into conoid test tube then added 5 ml of physiological saline, 1000rpm×10min, discard supernatant, saved 0.2-0.4ml;

    (4)     5μl of sperm suspensions was made into 2 pieces of smears in 1 cm, air drying;

    (5)     Two drops of PSA-FITC working solution at 4 ℃ for 1 hour;

    (6)     Washed slide with purified water and mounting with mounting agent;

    (7)     Observed sperm smear under microscope (400×) at 450-490nm.

    [Reference Results]

    AR<10% was abnormal.

    [Interpretation of the results Indentified]

    Acrosin Integrity (AI): fluorescent staining in more than half of sperm head is brighter and uniform; acrosin reaction (AR): fluorescence strap appears in equatorial zone or without fluorescein stain in acrosin zone; acrosin abnormality: all sperm except for the above two kinds of sperm.

    [Limitations of Test Method]

    Sperm have to be fresh.

    [Product Performance]

    Comparison with similar products overseas, Correlation coefficient0.654, P<0.0001


    Sperm should be fresh.

    [Medical Device Manufacturing Enterprise License Number] WSYJX 20070120

    [The registration Number of Medical Devices] GSYJX (Z) Z2011N1400171.

    [Standard Number]  YZB0037-2011.

    [Instruction Approval and Modified day] December 30st, 2011

    [Manufactured by]

    Anhui Anke Biotechnology (Group) Co., Ltd.


    Ankebio Buildings, 669, Changjiang Road, West ,Hefei, 230088, P.R. China.

    Tel: +86-551-65319890, 65318811


    Website: www.ankebio.com

    E-mail: info@ankebio.com