SPECIFICATION AND INSTRUCTION
Generic name: 千赢国际真人
20 samples per kit
PSA-FITC staining was used for the shape of sperm acrosin to evaluate acrosin function. The kit can be used for the evaluation of male fertility.
[Units of Kit]
1. Solution A PSA-FITC staining solution;
2. Solution B Nacl;
3. Solution C BWW;
4. Solution D mounting agent;
5. Slide 20 pieces
Stored at -20℃ for 10 months.
(1) One drop of fresh sperm is added into conoid test tube, then added 5 ml of physiological saline, 1000rpm×10min;
(2) Discard supernatant, 0.2-0.4ml, and added 1.2ml BWW culture solution, centrifuge tube beveled 45 at 37 ℃ for 1 hour;
(3) Erect centrifuge tube, imbibed 1 ml of upper straum into conoid test tube then added 5 ml of physiological saline, 1000rpm×10min, discard supernatant, saved 0.2-0.4ml;
(4) 5μl of sperm suspensions was made into 2 pieces of smears in 1 cm, air drying;
(5) Two drops of PSA-FITC working solution at 4 ℃ for 1 hour;
(6) Washed slide with purified water and mounting with mounting agent;
(7) Observed sperm smear under microscope (400×) at 450-490nm.
AR<10% was abnormal.
[Interpretation of the results Indentified]
Acrosin Integrity (AI): fluorescent staining in more than half of sperm head is brighter and uniform; acrosin reaction (AR): fluorescence strap appears in equatorial zone or without fluorescein stain in acrosin zone; acrosin abnormality: all sperm except for the above two kinds of sperm.
[Limitations of Test Method]
Sperm have to be fresh.
Comparison with similar products overseas, Correlation coefficient0.654, P<0.0001
Sperm should be fresh.
[Medical Device Manufacturing Enterprise License Number] WSYJX 20070120
[The registration Number of Medical Devices] GSYJX (Z) Z2011N1400171.
[Standard Number] YZB0037-2011.
[Instruction Approval and Modified day] December 30st, 2011
Anhui Anke Biotechnology (Group) Co., Ltd.
Ankebio Buildings, 669, Changjiang Road, West ,Hefei, 230088, P.R. China.
Tel: +86-551-65319890, 65318811